Purification of peptides and oligonucleotides: puriFlash® 5.125P
Purification of peptides and oligonucleotides: puriFlash® 5.125P
puriFlash® 5.125P
Optimized for your Flash and preparative purifications of complex mixtures of peptides and oligonucleotides.
Flow rate
250 mL/min
250 mL/min
Max. pressure
125 bar
125 bar
Components
- Control unit
- Pump
- UV detector
- Fraction collector
- Solvent tank
- 2 integrated column supports
- 2 integrated dry load mounts
Pump
- Flow rate: 250 mL/min
- Optimum flow rate for columns up to 80 mm internal diameter
- Max pressure: 125 bar
- Quaternary gradient valve
- Air bleed
- Wash discs and wash pump (circulates water to prevent crystalline deposits on the pump pistons)
- Solvent heater (under development)
Injection
- Injection mode: liquid or solid
- “Load & Go” technology: with automatic 10-way valve that allows the use of staggered dry load or loops for a more efficient injection
- “RFID Technology”: automatic detection of dry loads and injection loops
- 2 mL and 5 mL stainless steel injection loops
Detection
- UV detector 200-400 nm
- UV detector 200-800 nm (included in the Uvextended pack)
- “DAD” technology
- Multi-wavelength and scan over a band of 200 nm (or 600 nm with the UVextended pack)
- Spectral visualization and confirmation of purity
- 1.3 mm optical path cell
- Integrated ELSD detector (included in the iELSD pack)
Compatibility of columns and support
- Particle sizes: 10 μm, 15 μm, 25 μm, 30 μm, 50 μm
- .
- Format: All Flash and preparative columns up to an internal diameter of 80 mm (3”) are compatible
- .
- 2 integrated column supports (up to 3 column supports with the Multi pack)
- “RFID” technology: Automatic detection of the columns during insertion
Fraction collector
- Content:
– 3 racks
– 132 tubes (18 x 150 mm) - .
- Technology:
– Drainage system for the fraction collector
– Automatic cleaning of the fraction collector (to avoid cross-contamination with the previous purification)
– “RFID technology”: Automatic detection of the racks installed in the collector
Proposed racks
- Delivery includes 3 selected racks from the list below:
- ● Rack for tubes:
13 x 73 mm | 13 x 100 mm | 16 x 150 mm
18 x 150 mm | 21 x 150 mm | 25 x 150 mm
28 x 150 mm | 29.5 x 200 mm - ● Rack for Schott laboratory bottles 250 mL
- ● Rack for funnels
- .
- Position of the numbered tubes (to facilitate identification of the tubes)
Software and control unit
- Controlled by InterSoft X software
- Remote control
- 15“ touchscreen
- 8 USB ports
System optimization
- Test tubes 1.00 mm inside diameter
- Optical path length detection cell: 1.3 mm/55 μl
Safety
- Level detector for solvents
- Retention tank for solvents on the pump and valves with leak detector (pump, UV detector, valves and collector)
- Collector with drainage system
- “Fume Encloser” technology (allows the use of the cleaning system without extractor hood)
Communication
- Network cable RJ45
- Communication cable Sub-D9 male/female
- Communication cable Sub-D9 female/female
- 8 USB ports
Dimensions
