Purification of peptides and oligonucleotides: puriFlash® 5.125P

Purification of peptides and oligonucleotides: puriFlash® 5.125P

Purification of peptides and oligonucleotides puriFlash 5.125P purification

puriFlash® 5.125P

Optimized for your Flash and preparative purifications of complex mixtures of peptides and oligonucleotides.

Routine Flash purification puriFlash XS 520 Plus interchim
Flow rate
250 mL/min
Routine Flash purification puriFlash XS 520 Plus interchim
Max. pressure
125 bar
  • Small organic molecules 80% 80%
  • Impurity detection 80% 80%
  • Natural products 98% 98%
  • Peptides and oligonucleotides 98% 98%
  • Proteins and biopharmaceuticals 65% 65%
  • Isolation of metabolites 95% 95%
  • Trace enrichment 98% 98%

Components

  • Control unit
  • Pump
  • UV detector
  • Fraction collector
  • Solvent tank
  • 2 integrated column supports
  • 2 integrated dry load mounts

Pump

  • Flow rate: 250 mL/min
  • Optimum flow rate for columns up to 80 mm internal diameter
  • Max pressure: 125 bar
  • Quaternary gradient valve
  • Air bleed
  • Wash discs and wash pump (circulates water to prevent crystalline deposits on the pump pistons)
  • Solvent heater (under development)

Injection

  • Injection mode: liquid or solid
  • “Load & Go” technology: with automatic 10-way valve that allows the use of staggered dry load or loops for a more efficient injection
  • “RFID Technology”: automatic detection of dry loads and injection loops
  • 2 mL and 5 mL stainless steel injection loops

Detection

  • UV detector 200-400 nm
  • UV detector 200-800 nm (included in the Uvextended pack)
  • “DAD” technology
  • Multi-wavelength and scan over a band of 200 nm (or 600 nm with the UVextended pack)
  • Spectral visualization and confirmation of purity
  • 1.3 mm optical path cell
  • Integrated ELSD detector (included in the iELSD pack)

Compatibility of columns and support

  • Particle sizes: 10 μm, 15 μm, 25 μm, 30 μm, 50 μm
  • .
  • Format: All Flash and preparative columns up to an internal diameter of 80 mm (3”) are compatible
  • .
  • 2 integrated column supports (up to 3 column supports with the Multi pack)
  • “RFID” technology: Automatic detection of the columns during insertion

Fraction collector

  • Content:
    – 3 racks
    – 132 tubes (18 x 150 mm)
  • .
  • Technology:
    – Drainage system for the fraction collector
    – Automatic cleaning of the fraction collector (to avoid cross-contamination with the previous purification)
    – “RFID technology”: Automatic detection of the racks installed in the collector

Proposed racks

  • Delivery includes 3 selected racks from the list below:
  •  Rack for tubes:
    13 x 73 mm | 13 x 100 mm | 16 x 150 mm
    18 x 150 mm | 21 x 150 mm | 25 x 150 mm
    28 x 150 mm | 29.5 x 200 mm
  •  Rack for Schott laboratory bottles 250 mL
  •  Rack for funnels
  • .
  • Position of the numbered tubes (to facilitate identification of the tubes)

Software and control unit

  • Controlled by InterSoft X software
  • Remote control
  • 15“ touchscreen
  • 8 USB ports

System optimization

  • Test tubes 1.00 mm inside diameter
  • Optical path length detection cell: 1.3 mm/55 μl

Safety

  • Level detector for solvents
  • Retention tank for solvents on the pump and valves with leak detector (pump, UV detector, valves and collector)
  • Collector with drainage system
  • “Fume Encloser” technology (allows the use of the cleaning system without extractor hood)

Communication

  • Network cable RJ45
  • Communication cable Sub-D9 male/female
  • Communication cable Sub-D9 female/female
  • 8 USB ports

Dimensions

Purification of peptides and oligonucleotides puriFlash 5.125P flash purification

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