Ultra Performance Flash Purification: puriFlash® 5.020-5X

Ultra Performance Flash Purification: puriFlash® 5.020-5X

Ultra Performance Flash Purification puriFlash 5.020-5X

puriFlash® 5.020-5X

Optimized for your Ultra Performance Flash Purification

Routine Flash purification puriFlash XS 520 Plus interchim

Flow rate
300 mL/min

Routine Flash purification puriFlash XS 520 Plus interchim

Max. pressure
20 bar

  • Small organic molecules 98% 98%
  • Impurity detection 75% 75%
  • Natural products 85% 85%
  • Peptides and oligonucleotides 60% 60%
  • Proteins and biopharmaceuticals 25% 25%
  • Isolation of metabolites 25% 25%
  • Trace enrichment 98% 98%

Components

  • Control unit
  • Pump
  • UV detector
  • Fraction collector
  • Solvent tray with drainage system
  • 5 Integrated column holders

Pump

  • Flow rate range: 1 mL/min to 300 mL/min
  • Max. operational flow rate: 300 mL/min
  • Optimum flow rate for columns up to F0800 format
  • Flow rate accuracy: +/- 2 % over 20 to 200 mL/min & 5 to 16 bar
  • Flow rate repeatability: < 0.25
  • Flow rate linearity: 0.98 < linearity < 1
  • Pressure range: 0 to 20 bar
  • Max. operational pressure: 20 bar
  • Quaternary gradient valve
  • Gradient tolerance step: < 3.5 %
  • Air purge

Injection

  • Injection mode: liquid or solid
  • ”Load & Go” technology: with automatic 6-way valve which allows the use if a loop for an even more efficient injection
  • 2 mL Stainless Steel Loop with RFID tag

Detection

  • UV detector 200-400 nm
  • UV detector 200-800 nm (included in the pack-UVextended)
  • ”DAD” technology
  • Multi-wavelength and scan collection over a band of 200 nm (or 600 nm with the pack-UVextended)
  • Spectral visualization and confirmation of purity
  • Flow cell pathway: 0.3 mm / 40 µL
  • Integrated ELSD detector (included in the iELSD pack)

Column & holder compatibility

  • Normal phase (NP)
    Particle sizes: 15 µm, 25 μm, 30 μm, 40 μm , 50 μm
  • Reverse phase (RP)
    Particle sizes: 15 µm, 30 μm , 50 μm
  • .
  • Format: all Flash columns up to F0330 format & up to F0800 format on the front position (with optional Integrated column holder for F0800 flash column) are compatible
  • .
  • 5 Integrated column holders
  • .
  • RFID technology: automatic detection of the columns

Fraction collector

  • Capacity:
     3 racks
     132 tubes (18 x 150 mm)
  • .
  • Technology:
    Automatic cleaning of the fraction collector needle (to avoid cross-contamination with the previous purification)
  • RFID: automatic detection of the racks installed in the fraction collector

Proposed racks

  • Delivery includes a choice of 3 racks from the following list:
  •  Rack for tubes:
    13 x 73 mm | 13 x 100 mm | 16 x 150 mm
    18 x 150 mm | 18 x 180 mm | 21 x 150 mm
    25 x 150 mm | 28 x 150 mm | 29,5 x 200 mm
  • Rack for 250 mL Schott bottles
  • .
  • Available additional accessory:
  • Rack for funnels
  • .
  • Position of the numbered tubes (to facilitate identification of tubes)

Software & control unit

  • Control via InterSoft® X software included:
  • Genius, Artificial Intelligence
    ”Flash & Go”™ technology: compatible with our smartphone app ”TLC to Flash & Prep”
    ”Boost & Go”™ technology
  • Windows 10
  • Remote control
  • Touchscreen 15″
  • 8 USB ports
  • 2 RJ45 ports
  • Bluetooth/Wi-Fi key

System optimization

  • Tubing after pump : 1.6 mm internal diameter
  • Flow cell pathway: 0.3 mm / 40 µL

Safety

  • System light status
  • Solvent tray with drainage system
  • Level sensor for solvents and waste
  • Retention tray with leak detectors in the pump, column holders, UV detector, valve, fraction collector & iELSD (included in the Pack-iELSD)
  • ”Fume Enclosure” technology (allows the use of the purification system without a hood)

Materials in contact with solvents

  • ETFE
  • PTFE
  • 316 L Stainless steel
  • Hastelloy
  • Peek
  • Kalrez
  • Sapphire
  • Ruby
  • Kel-F
  • Zirconium
  • PPS
  • Nitronic 60
  • Borosilicate glass

Accessories box

  • Tubing with fitting & ferrules:
  • Tygon tubing for solvent tray drainage
    Level sensor tubing (x5)
    Suction tubes (x4)
    ETFE tubing 1/8″ selection valve to column C1/C2/C3/C4/C5 inlet (x5)
    ETFE tubing 1/8″ column C1 outlet to selection valve
    ETFE tubing 1/8″ column C2/C3 outlet to selection valve (x2)
    ETFE tubing 1/8″ column C4/C5 outlet to selection valve (x2)
    ETFE tubing 1/8″ selection valve to detector inlet
    Stainless steel adapter for injection (injection valve port 1)
    ETFE tubing 1/8″ injection valve port 6 to waste
    ETFE tubing 1/8″ fraction collector outlet to waste
    ETFE tubing 1/8″ draining for fraction collector
    2 mL Stainless steel loop with RFID tag
  • .
  • Accessories ELSD (included in the iELSD pack)
  • Reduction gear tubing 6 mm to tubing 4 mm
    White NYLON pneumatic tube length 3 m
    In-line filter 20 microns
    ETFE tubing line filter to detector
    ETFE tubing outlet column C1 to the filter online
  • .
  • Cables:
  • RFID cable (x4)
    USB/USB cable – unit control to fraction collector
    Power cord – screen to fraction collector
    HDMI cable – screen to unit control
    USB/MINI USB cable – screen to unit control
    LED’S cable – unit control to fraction collector
    Communication cable Sub-D9 male/female (1 m) – unit control to fraction collector
    Communication cable Sub-D9 male/female (0.5 m) – unit control to pump
    Communication cable Sub-D9 female/female (1 m) – unit control to UV detector
    Communication cable Sub-D9 female/female (1 m) – unit control to iELSD (included in the Pack-iELSD)
    Power cord – pump to fraction collector + unit control to fraction collector (x2)
    General power cable (model according to the country of destination)
  • .
  • Solvent tray
  • Clips P
  • Screw CHC M4x8
  • Grower M4 (x3)
  • Stylus with its clip
  • UV cell test
  • Rear fastening (x2)
  • Screws BHC 4×10 (x2)
  • UV trapdoor
  • ELSD trapdoor
  • Rack (x3)
  • Compliance report

Communication

  • Communication cable Sub-D9 male/female (1 m) – unit control to fraction collector
  • Communication cable Sub-D9 male/female (0.5 m) – unit control to pump
  • Communication cable Sub-D9 female/female (1 m) – unit control to UV detector
  • Communication cable Sub-D9 female/female (1 m) – unit control to iELSD (included in the Pack-iELSD)

Power supply

  • 110 V or 220 V

Dimensions & Weight

Ultra Performance Flash Purification: puriFlash® 5.020-5X
  • Weight : 49 kg

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