Purification of peptides and oligonucleotides: puriFlash® 5.250P

Purification of peptides and oligonucleotides: puriFlash® 5.250P

Purification of peptides and oligonucleotides puriFlash 5.250P
Purification of peptides and oligonucleotides puriFlash 5.250P

Configure your puriFlash® 5.250P

Optimized for your Flash and preparative purification of complex mixtures of peptides and oligonucleotides.

Flow rate
250 mL/min

Max. pressure
250 bar

Basic configuration

  • Components
    •  Control unit
    •  Pump
    •  UV detector
    •  Fraction collector
    •  Solvent container
    •  2 integrated column supports
    •  2 integrated dry load supports
    •  Accessories set
  • .
  • Pump
    •  Flow rate: 250 mL/min
    •  Optimum flow rate for columns up to a size of 60 mm inner diameter
    •  Max. pressure: 250 bar
    •  Quaternary gradient valve
    •  Air vent
    •  Wash discs and wash pump
    •  Solvent heater (under development)
  • .
  • Injection
    •  Injection mode: liquid or solid
    •  “Load & Go” technology: with automatic 10-way valve that allows the use of staggered dry load or 2 loops for even more efficient injection
    •  “RFID technology”: automatic detection of dry load and injection loops
    •  Stainless steel injection loops 2 mL and 5 mL
  • .
  • Detection
    •  UV detector 200-400 nm
    •  “DAD” technology
    •  Multi-wavelength and scan over a band of 200 nm (or 600 nm with the Uvextended pack)
    •  Spectral visualization and confirmation of purity
    •  1.3 mm optical path cell
  • .
  • Compatibility of columns and supports
    •  Particle sizes: 5 μm, 10 μm, 15 μm, 25 μm, 30 μm, 50 μm
    •  Size: Compatibility of all flash and preparative columns up to an inner diameter of 60 mm
    •  2 integrated column supports
    •  “RFID” technology: automatic detection of the columns during insertion
  • .
  • Fraction collector
    •  Content:
      – 3 racks
      – 132 tubes (18 x 150 mm)
    •  Technology:
      – Drainage system for the fraction collector
      – Automatic cleaning of the fraction collector (to avoid cross-contamination with the previous purification)
      – “RFID” technology: automatic detection of racks on the collector
  • .
  • Proposed racks
    •  Delivery includes 3 selected racks from the list below:
      – Rack for tubes:
      13 x 73 mm | 13 x 100 mm | 16 x 150 mm
      18 x 150 mm | 21 x 150 mm | 25 x 150 mm
      28 x 150 mm | 29.5 x 200 mm
      – Rack for Schott laboratory bottles 250 mL
      – Rack for funnels
      Position of the numbered tubes (to facilitate identification of tubes)
  • .
  • Software and control unit
    •  Control by InterSoft X software
    •  Remote monitoring
    •  Touchscreen 15″
    •  8 USB ports
  • .
  • System optimization
    •  Test tubes with 0.75 mm inner diameter
    •  Optical path length detection cell: 1.3 mm/55 μl
  • .
  • Safety
    •  Level detector for solvents
    •  Retention tank for solvents on the pump and valves with leak detectors (pump, UV detector, valves and collector)
    •  Collector with drainage system
    •  “Fume Encloser” technology (allows use of the purification system without the extractor hood)
  • .
  • Communication
    •  Network cable RJ45
    •  Communication cable Sub-D9 male/female
    •  Communication cable Sub-D9 female/female
    •  8 USB ports

Choose your option