Purification of peptides and oligonucleotides: puriFlash® 5.250P
Purification of peptides and oligonucleotides: puriFlash® 5.250P

puriFlash® 5.250P
Optimized for your Flash and preparative purification of complex mixtures of peptides and oligonucleotides.

Flow rate
125 mL/min

Max. pressure
250 bar
Components
- Control unit
- Pump
- UV detector
- Fraction collector
- Solvent container
- 2 integrated column supports
- 2 integrated dry load supports
Pump
- Flow rate: 125 mL/min
- Optimum flow rate for columns up to 60 mm inner diameter
- Max. pressure: 250 bar
- Quaternary gradient valve
- Air vent
- Wash discs and wash pump (allows circulation of water to prevent crystalline deposits on the pump piston)
Injection
- Injection mode: liquid or solid
- “Load & Go” technology: with automatic 10-way valve that allows the use of staggered dry load or loops for even more efficient injection
- “RFID technology”: automatic detection of dry load and injection loops
- Stainless steel injection loops 2 mL and 5 mL
Detection
- UV detector 200-400 nm
- UV detector 200-800 nm (included in the Uvextended pack)
- “DAD” technology
- Multi-wavelength and scan over a band of 200 nm (up to 600 nm with the Uvextended pack)
- Spectral visualization and confirmation of purity
- 1.3 mm optical path cell
- Integrated ELSD detector (included in the iELSD pack)
Compatibility of columns and supports
- Particle sizes: 5 μm, 10 μm, 15 μm, 25 μm, 30 μm, 50 μm
- .
- Size: Compatibility of all Flash and preparative columns up to an inner diameter of 60 mm
- .
- 2 integrated column supports (up to 3 column supports with the Multi pack)
- “RFID” technology: automatic detection of the columns during insertion
Fraction collector
- Content:
– 3 racks
– 132 tubes (18 x 150 mm) - .
- Technology:
– Drainage system for the fraction collector
– Automatic cleaning of the fraction collector (to avoid cross-contamination with the previous purification)
– “RFID technology”: automatic detection of the racks on the collector
Offered racks
- Delivery includes 3 selected racks from the list below:
- ● Rack for tubes:
13 x 73 mm | 13 x 100 mm | 16 x 150 mm
18 x 150 mm | 21 x 150 mm | 25 x 150 mm
28 x 150 mm | 29.5 x 200 mm - ● Rack for Schott laboratory bottles 250 mL
- ● Funnel racks
- .
- Position of the numbered tubes (to simplify labeling of the tubes)
Software and control unit
- Control by InterSoft X software
- Remote monitoring
- Touchscreen 15 “
- 8 USB ports
System optimization
- Test tubes of 0.75 mm inner diameter
- Optical path length detection cell: 1.3 mm/55 μl
Safety
- Level detector for solvents
- Retention tank for solvents on the pump and the valves, with leak detector (pump, UV detector, valves and collector)
- Collector with drainage system
- “Fume Encloser” technology (allows use the cleaning system without extractor hood)
Communication
- Network cable RJ45
- Communication cable Sub-D9 male/female
- Communication cable Sub-D9 female/female
- 8 USB ports
Dimensions
